Second-degree burns are skin injuries involving damage to the epidermis
and part of the dermis, requiring effective therapeutic management. Conventional
treatment such as silver sulfadiazine is widely used but may cause adverse effects
with prolonged application. Centella asiatica (L.) Urban leaves are known to
contain bioactive compounds that support wound healing. This study aimed to
evaluate the effectiveness of ethanol extract of Centella asiatica leaves and its film
forming spray formulation in the healing of second-degree burns, and to compare
their effectiveness with positive and negative control groups.
The study was initiated by preparing an ethanol extract of Centella asiatica
leaves followed by phytochemical screening to identify secondary metabolite
compounds. The extract was then formulated into a film forming spray and
evaluated for its physical characteristics. The wound healing activity was assessed
in vivo using an animal model with second-degree burns. Wound diameter
reduction and percentage of wound healing were observed on several observation
days. Data were analyzed using non-parametric statistical tests, namely the
Kruskal–Wallis test followed by the Mann–Whitney post hoc test.
The results showed that the ethanol extract of Centella asiatica leaves
contained flavonoids, saponins, and triterpenoids. The film forming spray
formulation exhibited good and stable physical characteristics. Statistically
significant differences among treatment groups were observed on day 4 and day
13. The film forming spray containing 25% ethanol extract demonstrated a higher
percentage of wound healing compared to the gel formulation with the same extract
concentration. In conclusion, the film forming spray delivery system enhances the
effectiveness of Centella asiatica ethanol extract in accelerating the healing
process of second-degree burns.